Composite

Part:BBa_K2598063:Design

Designed by: Matthew Gao   Group: iGEM18_UCAS-China   (2018-10-04)


sfGFP under CGG-regulated promoter pCGG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 757
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 42


Design Notes

Source

sfGFP is provided by Lou lab and pCGG is provided by Christopher A Voigt.

References

Fernandez-Rodriguez, J., Moser, F., Song, M. & Voigt, C. A. Engineering RGB color vision into Escherichia coli. Nature Chemical Biology 13, 706-708 (2017)

Segall-Shapiro, T. H., Meyer, A. J., Ellington, A. D., Sontag, E. D. & Voigt, C. A. A 'resource allocator' for transcription based on a highly fragmented T7 RNA polymerase. Mol Syst Biol 10, 742, (2014).